Table of Contents

PERSPECTIVE

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The limits of human microRNA annotation have been met
- Bastian Fromm,
- Xiangfu Zhong,
- Marcel Tarbier,
- Marc R. Friedländer,
- and Michael Hackenberg
RNA June 2022 28: 781-785; Published in Advance March 2, 2022, doi:10.1261/rna.079098.122

BIOINFORMATICS

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FISH-quant v2: a scalable and modular tool for smFISH image analysis
- Arthur Imbert,
- Wei Ouyang,
- Adham Safieddine,
- Emeline Coleno,
- Christophe Zimmer,
- Edouard Bertrand,
- Thomas Walter,
- and Florian Mueller
RNA June 2022 28: 786-795; Published in Advance March 28, 2022, doi:10.1261/rna.079073.121
OPEN ACCESS ARTICLE

ARTICLES

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Plasticity and conditional essentiality of modification enzymes for domain V of Escherichia coli 23S ribosomal RNA
- Josefine Liljeruhm,
- Margus Leppik,
- Letian Bao,
- Triin Truu,
- Maria Calvo-Noriega,
- Nicola S. Freyer,
- Aivar Liiv,
- Jinfan Wang,
- Rubén Crespo Blanco,
- Rya Ero,
- Jaanus Remme,
- and Anthony C. Forster
RNA June 2022 28: 796-807; Published in Advance March 8, 2022, doi:10.1261/rna.079096.121
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The interplay between translational efficiency, poly(A) tails, microRNAs, and neuronal activation
- Timothy J. Eisen,
- Jingyi Jessica Li,
- and David P. Bartel
RNA June 2022 28: 808-831; Published in Advance March 10, 2022, doi:10.1261/rna.079046.121
OPEN ACCESS ARTICLE
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Thermodynamic characterization of naturally occurring RNA pentaloops
- Md. Sharear Saon and
- Brent M. Znosko
RNA June 2022 28: 832-841; Published in Advance March 22, 2022, doi:10.1261/rna.078915.121
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Evolutionary divergence of Firre localization and expression
- Christian Much,
- Michael J. Smallegan,
- Taeyoung Hwang,
- Skylar D. Hanson,
- Gabrijela Dumbović,
- and John L. Rinn
RNA June 2022 28: 842-853; Published in Advance March 18, 2022, doi:10.1261/rna.079070.121
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Reversing the miRNA -5p/-3p stoichiometry reveals physiological roles and targets of miR-140 miRNAs
- Cameron Young,
- Melissa Caffrey,
- Christopher Janton,
- and Tatsuya Kobayashi
RNA June 2022 28: 854-864; Published in Advance March 24, 2022, doi:10.1261/rna.079013.121
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Ligands with polyfluorophenyl moieties promote a local structural rearrangement in the Spinach2 and Broccoli aptamers that increases ligand affinities
- Sharif Anisuzzaman,
- Ivan M. Geraskin,
- Muslum Ilgu,
- Lee Bendickson,
- George A. Kraus,
- and Marit Nilsen-Hamilton
RNA June 2022 28: 865-877; Published in Advance March 29, 2022, doi:10.1261/rna.079005.121
OPEN ACCESS ARTICLE
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ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5′ splice site motifs within nuclear speckles
- Eliza S. Lee,
- Harrison W. Smith,
- Eric J. Wolf,
- Aysegul Guvenek,
- Yifan E. Wang,
- Andrew Emili,
- Bin Tian,
- and Alexander F. Palazzo
RNA June 2022 28: 878-894; Published in Advance March 29, 2022, doi:10.1261/rna.079104.122
OPEN ACCESS ARTICLE

METHODS

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Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation
- Yusuke Kimura,
- Hironori Saito,
- Tatsuya Osaki,
- Yasuhiro Ikegami,
- Taisei Wakigawa,
- Yoshiho Ikeuchi,
- and Shintaro Iwasaki
RNA June 2022 28: 895-904; Published in Advance March 7, 2022, doi:10.1261/rna.079097.122
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Global SLAM-seq for accurate mRNA decay determination and identification of NMD targets
- Hanna Alalam,
- Jorge A. Zepeda-Martínez,
- and Per Sunnerhagen
RNA June 2022 28: 905-915; Published in Advance March 16, 2022, doi:10.1261/rna.079077.121
OPEN ACCESS ARTICLE

PERSPECTIVE

The limits of human microRNA annotation have been met

BIOINFORMATICS

FISH-quant v2: a scalable and modular tool for smFISH image analysis

ARTICLES

Plasticity and conditional essentiality of modification enzymes for domain V of Escherichia coli 23S ribosomal RNA

The interplay between translational efficiency, poly(A) tails, microRNAs, and neuronal activation

Thermodynamic characterization of naturally occurring RNA pentaloops

Evolutionary divergence of Firre localization and expression

Reversing the miRNA -5p/-3p stoichiometry reveals physiological roles and targets of miR-140 miRNAs

Ligands with polyfluorophenyl moieties promote a local structural rearrangement in the Spinach2 and Broccoli aptamers that increases ligand affinities

ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5′ splice site motifs within nuclear speckles

METHODS

Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation

Global SLAM-seq for accurate mRNA decay determination and identification of NMD targets

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