FIGURE 2.
Effect of nucleotide substitutions within h28 and hALT on translation activity in the cell. (A) Effects of mutations at positions 1532–1534 of the 16S rRNA, as indicated. A two-tailed t-test was used to evaluate differences from WT. Uncorrected P-values: (*) P < 0.05; (**) P < 0.005; (***) P < 0.005. (B) Effects of mutations at positions 922–923, 1393–1395, and 1532–1533, as indicated. Whether h28 and hALT contain a mismatch (red X) or are fully complementary (green ✓) is indicated for each construct. Data represent the mean ± SEM from four or more biological replicates. Raw data are given in Supplemental Table S1. (WT) Wild type, (VC) vector control.
