ADARs mediate distinct RNA editing activity and gene regulation in the Caenorhabditis elegans germline

ADR-2-dependent gene expression changes are buffered at the translational level. (A) Quadrant scatter plot showing the fold change (log₂) of germline expression (x-axis) and TRAP ribosome association (y-axis) in adr-2(-) germlines compared to wild type. Gray points denote genes with no significant change in either analysis. Red squares indicate a significant change in mRNA expression only (DESeq2 P_adj ≤ 0.05, log₂ fold change |≥0.5|) in adr-2(-) compared to wild type, and green triangles indicate a significant change in ribosome association only (DESeq2 P_adj ≤ 0.05, log₂ fold change |≥0.5|) in adr-2(-) compared to wild type. Blue diamond indicates a significant change in both expression and ribosome association (adr-2 only). DESeq2 analyses were performed with three biological replicates. Dotted lines represent log₂ fold change significance cutoff. (B) Log₂ fold change mRNA expression (red circles) and log₂ fold change germline ribosome association (black squares) in adr-2(-) germlines compared to wild type for genes showing significant changes in mRNA expression (DESeq2 Padj ≤ 0.05, log₂ fold change |≥0.5|).