ADR-2 edits distinct targets in the germline and is regulated by ADR-1. (A) Genic distribution of high-confidence germline editing sites identified in RNA-seq data from germlines of wild-type animals. (B) Comparison of edited sites (left) and edited genes (right) between adult germline samples (this study, green), adult neural samples (Rajendren et al. 2021) (blue), and adult whole worm samples (Schiksnis et al. 2024) (yellow). (C) Two Sample Logo analyses comparing flanking nucleotides of germline and/or neural editing sites to flanking nucleotides of nearby random adenosines. Favored (above) and disfavored (below) nucleotides are shown on the x-axis. Letter height corresponds to the difference between the positive and background inputs. Statistical significance was determined by a two-sample t-test, P < 0.05, with no Bonferroni correction. (D) ADR-1-regulated germline editing sites. Percent editing at individual germline editing sites for three independent biological replicates of RNA-seq data from wild-type and adr-1(-) germlines.
