ADARs are abundantly expressed in the young adult C. elegans germline. (A) Relative mRNA expression of adr-2 (left) and adr-1 (right) in wild-type, germline-lacking glp-4 temperature-sensitive mutant, and adr null mutant young adult animals raised at the restrictive temperature of 25°C. adr expression is normalized to the housekeeping gene gpd-3. Data represent three biological replicates. Significance determined via one-way ANOVA, (****) P ≤ 0.0001. (B) Representative confocal images of young adult (left) and L4 (right) extruded germlines stained for DAPI (top, cyan), V5::ADR-1 (top middle, green), and 3xFLAG::ADR-2 (bottom middle, red). (Bottom panel) Signals of ADR-1 and ADR-2 panels merged. Dotted lines denote proximal germline region containing oocytes (young adult) or sperm (L4). Each image includes two gonad arms and the intestine from the same animal. Scale bars, 50 μm.
