Codon bias shapes bacterial small RNA binding sites within protein-coding sequences

TABLE 1.

Prime positions in the binding sites of sRNAs

sRNAa Number of targets Length of binding site Prime position (within binding site on target) Number of binding sites with prime position in N1 Number of binding sites with prime position in N2 Number of binding sites with prime position in N3 Nucleotide in sRNA Nucleotide in target Coincidence with frequent codon? (N3)b Percentage of frequent codons (N3)c
CyaR 98 15 11 34 8 56 C 98G 47 0.8392
FliX 5 11 7 0 1 4 A 5U 0 0
MicA 14 15 10 6 2 6 G 14C 6 1
UhpU 13 8 6 3 2 8 G 13C 7 0.875
DsrA 6 11 7 1 4 1 C 6G 1 1
RprA 19 9 3 5 10 4 G 17C, 2U 1 0.25
RprA 19 9 6 5 10 4 G 18C, 1U 4 1
ArcZ 87 13 6 52 7 28 G 1A, 85C, 1U 20 0.7142
ArcZ 87 13 8 7 28 52 C 1A, 85G, 1U 50 0.9615
CpxQ 44 15 6 4 29 11 A 44U 3 0.2727
CpxQ 44 15 7 11 4 29 C 44G 19 0.6551
SdhX 7 11 4 1 4 2 A 7U 1 0.5
SdhX 7 11 5 2 1 4 C 7G 4 1
GadF 7 8 4 1 0 6 C 7G 6 1
GadF 7 8 5 6 1 0 A 7G 0 0
Spf 5 8 1 1 3 1 U 5A 1 1
Spf 5 8 2 1 1 3 U 5A 3 1
Spf 5 8 7 1 3 1 G 5C 0 0
RyhB 55 11 3 40 8 7 G 55C 1 0.1429
RyhB 55 11 4 7 40 8 A 55U 0 0
RyhB 55 11 5 8 7 40 C 55G 40 1
MgrR 14 8 1 7 7 0 C 14G 0 0
MgrR 14 8 3 7 0 7 U 14A 0 0
MgrR 14 8 4 7 7 0 A 14U 0 0
MgrR 14 8 5 0 7 7 C 14G 7 1
MgrR 14 8 6 7 0 7 C 14G 7 1
  • asRNAs are ordered by the number of prime positions they have.

  • bNumber of targets in which the prime positions that align with a third codon position coincide with the frequent codon.

  • cFraction of targets in which the prime positions that align with a third codon position coincide with the frequent codon.

This Article

  1. RNA 32: 568-583