Possible alignments of prime positions with codon positions. Possible adjustments of RyhB binding sites in reference to the reading frames of the targets are demonstrated. For RyhB, three prime positions were identified (Fig. 1) and are underlined. (A) Example of RyhB interaction with a target where the first prime position aligns with position N3 of a codon, and the next two prime positions align with positions N1 and N2 of the next codon. (B) Example of RyhB interaction with a target where the two prime positions align with positions N2 and N3 of a codon, and the last prime position aligns with position N1 of the next codon. (C) Example of RyhB interaction with a target where the three prime positions are adjusted with the reading frame.
