Probing the epitranscriptome and RNA damage with nanopore direct RNA sequencing

TABLE 1.

Values for copies and modification density in tRNA, rRNA, and mRNA in human and E. coli cells (Milo et al. 2009)

RNA type %Mass/cell Copies/cell #Sequences (#seq. variants) Ave. length #Mods Mod density
tRNA human ∼10 105 ∼50 cytosol; 22 mitochondria 80   13 1 in 6
rRNA E. coli ∼80 104 3 (16) 4500 (total value)   36 1 in 120
rRNA human cytosolic ∼80 107 4 (∼250) 6800 (total value)   230 1 in 30
rRNA human mitochondrial ∼5 105 2 (100s) 2500 (total value)   10 1 in 250
mRNA human ∼4 105 104–105 ∼2000 ∼10 1 in 100

This Article

  1. RNA 32: 412-427