Probing the epitranscriptome and RNA damage with nanopore direct RNA sequencing

Monitoring RNA polymerization with competing nucleotide triphosphates using nanopore DRS. (A) Schematic of in vitro transcription for monitoring insertion yields of competing nucleotide triphosphates. (B) The insertion yields of m¹ΨTP when competed against UTP for the active site of T7 RNA polymerase. (C) Sequence comparison of T7 and SP6 RNA polymerases to display differences in active site residues. (D) The insertion yields of m¹ΨTP when competed against UTP for the active site of SP6 RNA polymerase. The figure was adapted from our prior work (Fleming and Burrows 2023).