FIGURE 4.
The helicase motor protein in the ONT sequencer is a sensor of base pair integrity for RNA modification sequencing. (A) Structure for m4Cm1402 in the E. coli 16S rRNA. (B) Schematic to illustrate the RNA sequence distance between the nanopore constriction zone and the helicase active site. (C) Pairwise dwell-time population analyses between E. coli rRNA and a synthetic 16S rRNA sequence without modifications to locate sites that differ with a statistical difference. The figure was reconstructed from our prior report (Fleming et al. 2023a).
