FIGURE 6.
Native gel electrophoresis of Arm B with various divalent metals and annealing conditions. Arm B was prepared by snap-cooling (A), slow cooling (B), or native isolation (C) in filtration buffer (see Materials and Methods) before incubation with divalent metals. Asterisk denotes dimer favored during slow cooling. Black line through gels is for reference only; magnesium titration and incubation for other divalent metals was run on the same gel for each panel. RNA was generated using FPLC purification, and each gel was run in biological duplicate (kit-purified of snap- and slow-cooled Arm B run in triplicate, Supplemental Fig. S10B).
