Structural analysis of the lncRNA SChLAP1 reveals protein binding interfaces and a conformationally heterogenous retroviral insertion

Native gel electrophoresis of Arm A and Arm B with varying annealing protocols and magnesium concentrations. RNA was snap- or slow-cooled in filtration buffer (50 mM K-HEPES pH 7.5, 150 mM KCl, 0.1 mM EDTA) before incubation with the given Mg²⁺ concentration. Asterisk denotes putative Arm B dimer favored in slow cooling. RNA was purified using the “kit-purified” approach. Arm A gel is representative of two biological replicates, and Arm B gel is representative of three biological replicates.