TABLE 3.
Kinetic rates measured for transitions between the nonrotated conformation and the rotated conformation
| Oligo | P-site/A-site tRNA | #τNR | k1 (sec−1) | #τR | k−1 (sec−1) |
|---|---|---|---|---|---|
| +10 | tRNAfMet/vacant | 1306 | 0.72 ± 0.01 | 1263 | 0.51 ± 0.01 |
| +11 | tRNAfMet/vacant | 712 | 0.75 ± 0.01 | 761 | 0.58 ± 0.01 |
| +12 | tRNAfMet/vacant | 2431 | 0.81 ± 0.01 | 2374 | 0.41 ± 0.01 |
| +13 | tRNAfMet/vacant | 1333 | 0.90 ± 0.02 | 1369 | 0.39 ± 0.01 |
| +14 | tRNAfMet/vacant | 2356 | 0.83 ± 0.01 | 2306 | 0.30 ± 0.00 |
| +15 | tRNAfMet/vacant | 1864 | 0.71 ± 0.01 | 1764 | 0.32 ± 0.01 |
| +16 | tRNAfMet/vacant | 1469 | 0.83 ± 0.01 | 1435 | 0.32 ± 0.01 |
| Mean rates | 0.79 ± 0.06 | 0.41 ± 0.1 | |||
| +10 | tRNAfMet/NAc-Phe-tRNAPhe | 3181 | 0.78 ± 0.01 | 3041 | 0.43 ± 0.01 |
| +11 | tRNAfMet/NAc-Phe-tRNAPhe | 1525 | 0.75 ± 0.01 | 1450 | 0.43 ± 0.01 |
| +12 | tRNAfMet/NAc-Phe-tRNAPhe | 2831 | 0.69 ± 0.01 | 2775 | 0.47 ± 0.01 |
| +13 | tRNAfMet/NAc-Phe-tRNAPhe | 2054 | 0.73 ± 0.01 | 2106 | 0.34 ± 0.01 |
| +14 | tRNAfMet/NAc-Phe-tRNAPhe | 1627 | 1.05 ± 0.02 | 1632 | 0.50 ± 0.01 |
| +15 | tRNAfMet/NAc-Phe-tRNAPhe | 3957 | 0.88 ± 0.02 | 4237 | 0.42 ± 0.01 |
| +16 | tRNAfMet/NAc-Phe-tRNAPhe | 3110 | 0.59 ± 0.01 | 3129 | 0.32 ± 0.01 |
| Mean rates | 0.78 ± 0.14 | 0.42 ± 0.06 |
-
(#τNR) Total number of dwell times in the nonrotated (NR) state. (k1) Forward rate of transitioning from nonrotated state to rotated state. (#τR) Total number of dwell times in the rotated (R) state. (k−1) Reverse rate of transitioning from rotated to nonrotated state.
