TABLE 1.

Statistical analysis for all tested ribosomal complexes

Oligo	Figure	% Hyperrotated^a	% Rotated^b	% Nonrotated^c	% Fluctuating traces^d	Total number of molecules
tRNA^fMet
10	A	—	73	27	52.2	387
11	B	43	38	19	28.3	315
12	C	26	51	23	41.8	368
13	D	—	70	30	53.8	359
14	E	—	73	27	54.6	346
15	F	—	70	30	48.3	356
16	G	—	74	26	49.8	297
dnaX SL	H	39	56	6	10.3	571
tRNA^fMet and N-Ac-Phe-tRNA^Phe
10	A	—	60	40	46.1	356
11	B	13	52	36	35.8	265
12	C	19	53	28	32.7	376
13	D	—	59	41	45	362
14	E	—	64	36	47.3	311
15	F	—	65	35	45.5	380
16	G	—	62	38	46.7	321
dnaX SL	H	25	58	17	10.7	457

^aPercentage of molecules in the hyperrotated state compared to the total number of molecules as calculated from the fitted histogram.
^bPercentage of molecules in the rotated state compared to the total number of molecules as calculated from the fitted histogram.
^cPercentage of molecules in the nonrotated state compared to the total number of molecules as calculated from the fitted histogram.
^dPercentage of the traces showing at least a single transition event between hyperrotated, rotated, and nonrotated states out of the total number of molecules.

The influence of downstream structured elements within mRNA on the dynamics of intersubunit rotation in ribosomes