RNase L produces tRNA-derived RNAs that contribute to translation inhibition

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FIGURE 1.
FIGURE 1.

RNase L-mediated tRNA cleavage induced by poly(I:C) transfection. (A, B) Poly(I:C) transfection induces tRNA cleavage in U2OS/A549 cells. (A) SYBR Gold staining and (B) northern blotting for tRNAHis-GTG, tRNAPro-TGG, and tRNAGln-CTG. (C, D) RNASEL knockdown decreases the amount of poly(I:C)-induced tDRs in A549 cells. (C) SYBR Gold staining and (D) northern blotting for tRNAHis-GTG and tRNAPro-TGG. RNase L expression levels evaluated by western blotting are also shown. Red arrowheads indicate 5′-tDRs generated by RNase L-mediated cleavage.

This Article

  1. Published in Advance April 15, 2025, doi: 10.1261/rna.080419.125 RNA 31: 961-972

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