TABLE 3.
SL5 mRNA translation in vitro
| mRNA variant | Uncapped | Capped |
|---|---|---|
| WT | 1.00a | 1.00a |
| A266G | 0.35 ± 0.03 | 0.28 ± 0.02 |
| SL5aGAAA | 0.97 ± 0.10 | 0.82 ± 0.06 |
| SL5bGAAA | 1.12 ± 0.09 | 0.99 ± 0.11 |
| G256U/A257U/A258U | 0.94 ± 0.06 | 0.55 ± 0.09 |
| ΔG174 | 0.93 ± 0.10 | 0.77 ± 0.08 |
| ΔU211 | 1.12 ± 0.11 | 0.99 ± 0.12 |
| Δ235A | 0.92 ± 0.06 | 0.87 ± 0.04 |
| A187C | 0.86 ± 0.08 | 0.73 ± 0.07 |
| C186A | 1.02 ± 0.11 | 1.04 ± 0.10 |
| G219U | 1.02 ± 0.04 | 1.09 ± 0.05 |
| G219C | 1.12 ± 0.17 | 0.93 ± 0.27 |
| U182C | 1.06 ± 0.13 | 1.05 ± 0.30 |
| A263G | 0.94 ± 0.16 | 0.88 ± 0.14 |
| U262G | 1.24 ± 0.13 | 1.31 ± 0.07 |
| A253G | 1.12 ± 0.10 | 1.16 ± 0.19 |
| A253G,U262C | 1.09 ± 0.09 | 0.95 ± 0.04 |
| G252A | 1.30 ± 0.11 | 1.32 ± 0.04 |
| C228U | 1.20 ± 0.17 | 1.18 ± 0.18 |
| G252A,C228U | 1.05 ± 0.15 | 1.08 ± 0.04 |
| G227A | 1.17 ± 0.26 | 1.09 ± 0.15 |
| C183U | 1.25 ± 0.12 | 1.21 ± 0.10 |
| G227A,C183U | 1.10 ± 0.12 | 1.03 ± 0.17 |
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(SL5) Stem–loop 5, (SD) standard deviation, (WT) wild type.
-
aValues for uncapped mRNAs were normalized by the WT uncapped mRNA, and values for capped mRNAs were normalized by the WT capped mRNA. For all values, mean ± SD, n = 6.
