Crystallographic and cryoEM analyses reveal SARS-CoV-2 SL5 is a mobile T-shaped four-way junction with deep pockets

TABLE 2.

Summary of cryoEM data collection statistics

Data collection
Source location NICE (Bldg 37)
Microscope Krios G1
Detector Gatan BioQuantum K3 camera
Date 2022-10-03
Grid type Quantifoil R1.2/1.3 400 mesh
Magnification 105,000
Voltage (kV) 300
Electron exposure (e/Å2) 50
Frames (no.) 50
Defocus range (µm) −1.2 to 3.0
Pixel size (Å) 0.83
Initial micrographs (no.) 10,519
Curated micrographs (no.) 9297
Initial particle images (no.) 3,384,252
Final particle images (no.) 111,514
Map resolution (Å) (FSC = 0.143) 4.56
EMPIAR deposition EMPIAR-12428
  • (cryoEM) Cryogenic sample electron microscopy.

This Article

  1. RNA 31: 949-960