TABLE 1.
Summary of crystallographic statistics
| Data collection | Data set 1 (Cs+) | Data set 2 |
|---|---|---|
| Wavelength (Å) | 1.8917 | 1.5998 |
| Space group | P 32 2 1 | P 32 2 1 |
| Unit cell dimensions | ||
| a, b, c (Å) | 96.0, 96.0, 114.4 | 96.7, 96.7, 114. 3 |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 |
| Resolution (Å) | 48–3.51 (3.64–3.51)a | 47.36–3.33 (3.45–3.33) |
| No. reflections | 7981 (780) | 9443 (905) |
| Rmerge | 0.100 (4.967) | 0.072 (3.508) |
| <I>/<σ(I)> | 17.3 (0.7) | 20.9 (0.9) |
| CC1/2 | 0.996 (0.368) | 0.997 (0.435) |
| Completeness (%) | 99.6 (97.1) | 99.9 (99.1) |
| Redundancy | 18.5 (17.2) | 19.4 (20.1) |
| Refinement | ||
| Resolution (Å) | 48–3.51 | 47.36–3.33 |
| No. reflections | 7954 (780) | 9432 (902) |
| No. reflections (test set) | 792 (10%) | 953 (10%) |
| Rwork/Rfree (%) | 0.174/0.214 | 0.158/0.204 |
| No. atoms | ||
| RNA | 2129 | 2136 |
| Ions, waters | 44 | 35 |
| B-factors | ||
| RNA (Å2) | 207.4 | 206.4 |
| Ions, waters (Å2) | 242.1 | 210.7 |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.005 | 0.007 |
| Bond angles (°) | 1.17 | 1.36 |
| Coordinate precision (Phenix), Å | 0.59 | 0.56 |
| Protein Data Bank code | 9E90 | 9E9Q |
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aValues in parentheses correspond to highest resolution shells.
