Gold standard to verify structured ncRNA function. (A) Structure mutation design of SMDesigner. Circles mark base pairs selected to make mutations: black for wild-type (WT) nucleotides and red for mutated nucleotides, which make disrupted and compatible mutations. (B) Analysis overview for structure assessment. Green tick mark indicates an assay that confirms the importance of the structure for the assessed function; red X indicates that the importance of structure for the function is not confirmed and likely infirmed. In most cases where no function is observed for WT (right), even after optimization, the mutants designed by SMDesigner will not help assess the importance of the structure for the RNA's function.
