tRNA pseudouridine synthase D (TruD) from Thermus thermophilus modifies U13 in tRNA^Asp, tRNA^Glu, and tRNA^Gln and U35 in tRNA^Tyr

Nucleoside analysis of tRNA fractions from WT and ΔtruD strains revealed that overall modification levels are comparable. (A–D) About 500 pmol tRNA were digested to mononucleoside and analyzed by HPLC. Modified nucleosides were monitored with UV absorbance (A and B) at 254 nm and (C and D) at 330 nm. The peaks were assigned as described in our previous paper (Tomikawa et al. 2010; Ishida et al. 2011; Yamagami et al. 2016; Sugio et al. 2023). The experiments were independently performed three times (n = 3).