tRNA pseudouridine synthase D (TruD) from Thermus thermophilus modifies U13 in tRNA^Asp, tRNA^Glu, and tRNA^Gln and U35 in tRNA^Tyr

Bacterial TruD pseudouridylates U13 in tRNA. (A) Superposition of structures for yeast Pus7 (white blue) and E. coli TruD (orange) in PyMOL. The three insertion domains in Pus7 are labeled as I, II, and III. (B) Close-up of the catalytic cores in Pus7 (white blue) and E. coli TruD (orange) demonstrating their structural similarity. Conserved amino acid residues for E. coli TruD are compared to those of Pus7 (in brackets). (C) Secondary structure of tRNA^Asp-GUC in T. thermophilus with modified positions numbered. (D) TruD pseudouridylates U13 in tRNA.