FUBP1 and MYEF2 regulate the expression of SPAs in PA1 cells. (A) Knockdown of FUBP1 impairs the expression of SPAs. (Left) Knockdown efficiency of FUBP1 analyzed by WB. (Right) Expression levels of pre-snrpn, snrpn, pre-SPAs, and SPAs detected by RT-qPCR. (B) Knockdown of MYEF2 impairs the expression of SPAs. (Left) Knockdown efficiency of MYEF2 analyzed by WB. (Right) Expression levels of pre-snrpn, snrpn, pre-SPAs, and SPAs detected by RT-qPCR. (C) Representative images showing the dampened size of PWS bodies (SPAs’ area) and reduced expression of SPAs in the FUBP1 knockdown PA1 cells compared to the scramble PA1 cells. Scale bar, 5 μm (uncropped) and 1 μm (cropped). (D) Knockdown of FUBP1 dampens the size of the PWS bodies in PA1 cells. n = 21 scramble and n = 24 FUBP1 knockdown cells are evaluated. (E) Representative images showing the reduced expression of pre-snrpn in the FUBP1 knockdown PA1 cells compared to the scramble PA1 cells. Scale bar, 5 μm. (F) Knockdown of FUBP1 leads to the reduced expression of SPAs and pre-snrpn in PA1 cells. For evaluating SPA1 expression, n = 27 scramble and n = 26 FUBP1 knockdown cells are used. For evaluating SPA2 expression, n = 18 scramble and n = 17 FUBP1 knockdown cells are used. For evaluating pre-snrpn expression, n = 16 scramble and n = 24 FUBP1 knockdown cells are used. (G) Representative images showing the dampened size of PWS bodies (SPAs’ area) and reduced expression of SPAs in the MYEF2 knockdown PA1 cells compared to the scramble PA1 cells. Scale bar, 5 μm (uncropped) and 1 μm (cropped). (H) Knockdown of MYEF2 dampens the size of the PWS bodies in PA1 cells. n = 20 scramble and n = 21 MYEF2 knockdown cells are evaluated. (I) Representative images showing the reduced expression of pre-snrpn in the MYEF2 knockdown PA1 cells compared to the scramble PA1 cells. Scale bar, 5 μm. (J) Knockdown of MYEF2 leads to the reduced expression of SPAs and pre-snrpn in PA1 cells. For evaluating SPA1 expression, n = 18 scramble and n = 22 MYEF2 knockdown cells are used. For evaluating SPA2 expression, n = 17 scramble and n = 30 MYEF2 knockdown cells are used. For evaluating pre-snrpn expression, n = 20 scramble and n = 23 MYEF2 knockdown cells are used. (K) Knockdown of HNRNPM does not affect the expression of SPAs. (Left) Knockdown efficiency of HNRNPM analyzed by WB. (Right) Expression levels of pre-snrpn, snrpn, pre-SPAs, and SPAs detected by RT-qPCR.
