TABLE 2.
Summary of Ala misincorporation identified by MS/MS
| tRNAAla gene | tRNA variant | mCherry residue | Maximal −10*log(P) | Ion count |
|---|---|---|---|---|
| CGC-1-1 | WT | n.d. | n.d. | n.d. |
| G35T | E6A | 37.61 | 1 A/7 E | |
| E7A | 61.66 | 3 A/5 E | ||
| E15A | 56.24 | 2 A/9 E | ||
| E24A | 47.29 | 2 A/14 E | ||
| E31A | 55.76 | 2 A/14 E | ||
| E33A | 70.03 | 10 A/13 E | ||
| E35A | 71.31 | 8 A/15 E | ||
| E37A | 70.34 | 6 A/17 E | ||
| E39A | 53.85 | 3 A/20 E | ||
| E94A | 44.53 | 1 A/5 E | ||
| E149A | 89.87 | 4 A/4 E | ||
| E153A | 76.69 | 2 A/6 E | ||
| E158A | 73.82 | 1 A/8 E | ||
| E165A | 53.03 | 1 A/5 E | ||
| E181A | 79.41 | 3 A/4 E | ||
| E233A | 47.32 | 1 A/2 E | ||
| TGC-4-1 | G35A | V21A | 78.34 | 1 A/8 V |
| V27A | 67.23 | 1 A/14 V | ||
| V53A | 45.83 | 2 A/14 V | ||
| V78A | 57.38 | 2 A/10 V | ||
| V140A | 83.00 | 8 A/20 V | ||
| V192A | 61.57 | 1 A/10 V | ||
| AGC-8-1 | C36T | T46A | 63.29 | 2 A/10 T |
| T48A | 39.53 | 1 A/11 T | ||
| T54A | 67.09 | 5 A/13 T | ||
| T132A | 70.79 | 1 A/17 T | ||
| T145A | 79.14 | 3 A/17 T | ||
| T184A | 77.83 | 5 A/15 T | ||
| T185A | 75.36 | 4 A/16 T | ||
| T207A | 58.15 | 2 A/5 T | ||
| T214A | 59.46 | 2 A/5 T | ||
| T228A | 37.72 | 1 A/5 T |
-
(n.d.) Not detected.
