Let-7 miRNAs can repress AGO1 expression in mESCs. (A) Ectopic expression of let-7a in mESCs. The let-7a levels were determined by qRT-PCR, and U6 RNA was used for normalization. (B) Western blotting in the WT mESCs expression a vector and let-7a miRNAs. (C) qRT-PCR quantification of miRNAs in the WT and the let-7Δ mESCs. U6 RNA was used for normalization. (D) Western blotting in the WT and the let-7Δ mESCs. (E) Western blotting in the WT and the let-7Δ mESCs expressing either a vector or AGO2. Data information: In B, D, and E, representative western blots are shown, and the quantifications represent the means (±SD) of three independent experiments. GAPDH levels were used for normalization in calculating the relative protein levels. One-way ANOVA was used to determine the significance of the difference, (*) P < 0.05, (n.s.) not significant (P > 0.05).
