FIGURE 1.
AGO2 represses AGO1 in mESCs. (A) Western blotting in the WT, AGO1Δ, and AGO2Δ mESCs cultured in the 15% FBS + Lif medium. (B) Western blotting in the WT mESCs expressing a vector, WT AGO2, and an RNA-binding mutant AGO2 in the 15% FBS + Lif medium. Data information: In A and B, representative western blots are shown, and the quantifications represent the means (±SD) of three independent experiments. GAPDH levels were used for normalization in calculating the relative protein levels. One-way ANOVA was used to determine the significance of the difference, (*) P < 0.05.
