Meta-analysis of the human SG transcriptome. (A) Overview of data sets included in the meta-analysis. Techniques to isolate SG RNAs: bp, biochemical purification = differential centrifugation > immunoprecipitation; bp*, immunoprecipitation; pl, biotin-based proximity labeling > streptavidin pulldown; (pic) photo-isolation chemistry; (fanci) fluorescence-activated nonmembrane condensates isolation. (B) Most represented RNA types among total SG-enriched RNAs. (C) Number of SG RNAs grouped by CS. (D) Number of SG RNAs per consensus category: low, CS < 4; medium, 4 ≤ CS ≤ 9; high, CS > 9. “Not SG enriched” category includes all human-annotated genes except SG-enriched lists. (E) Length in nucleotides and AU content of SG RNAs (canonical transcripts) per consensus category, considering 5′ UTR, CDS, and 3′ UTR. Median values are reported. P-values are based on the Wilcoxon one-tailed test.
