TABLE 1.
Kd values of the protein/RNA complexes studied
| Complex | Kd (µM)a | Figure |
|---|---|---|
| apo-MmTtuI/MmtRNALys | 0.63 ± 0.1 | Figure 2B |
| apo-MmTtuI/MmtRNALys +AMP-CPP | 0.55 ± 0.08 | Figure 2C |
| apo-MmTtuI/MmtRNALys no Mg2+ | 2.2 ± 0.4 | Supplemental Figure S3B |
| apo-MmTtuI/TLYS41A | 1.9 ± 0.1 | Supplemental Figure S3A |
| apo-MmTtuI/TLYS41A no Mg2+ | 7.2 ± 1.4 | Supplemental Figure S3A |
| holo-MmTtuI/MmtRNALys | 0.5 ± 0.13 | Figure 2E |
| holo-MmTtuI/MmtRNALys + AMP-CPP | 0.7 ± 0.25 | Figure 2F |
| apo-EcTtcA/EctRNAArg | 3.5 ± 0.6 | Figure 4 |
| holo-EcTtcA/EctRNAArg | 4 ± 1.5 | Figure 4 |
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aThe standard deviation values were calculated from three and two replicates for the experiments performed with the apo- and holo-proteins, respectively.
