NSUN7 binds elongated spermatid RNAs. (A) Distribution of NSUN7 cross-link sites (FC > 2, P < 0.05) between transcript types. (B) Distribution of NSUN7 cross-link sites (FC > 2, P < 0.05) between mRNA parts. (C) Logo of consensus binding motif of NSUN7. (D) Coverage scatter plot of cross-linked and precipitated RNAs for the HA-NSUN7 and the wild-type control mice. Red dots correspond to the transcripts characteristic to elongated spermatids according to the scRNA-seq data, green dots correspond to other transcripts. Only RNAs that passed a statistical significance threshold (P < 0.05) are shown. (E) Correlation between the efficiency of RNA cross-linking to the NSUN7 as determined by CLIP-seq with the difference RNA abundance in wild-type and Nsun7i2/i2 mice as determined by RNA-seq. (F) Comparison of the mRNA levels of NSUN7 potential binding targets in wild-type and Nsun7i2/i2 mice. T-test was used, stars indicate P-value: (*) P < 0.05, (***) P < 0.001.
