RNA-templated RNA extension during DNA-templated transcription. (A) Schematic of experiment assessing RNA-templated RNA extension during DNA-templated transcription. The nontranscribed portion of the DNA template is in gray, the transcribed portion of the DNA template and the RNA transcript are in purple, and the RNA extension is in red. (B) RNA-seq analysis of the length distribution of RNA extensions during DNA-templated transcription. (C) An example extension product of the run-off transcript and a model for its formation. (D) Table of the five most abundant extension products. In C and D, colors for the extension sequences are as in Figure 3B. Reaction conditions were as described in Materials and Methods.
