EF-Tu function depends on the SRL. (A) Evaluation of 70S ICs formed with isolated or reconstituted (recon) 50S particles (as indicated), as determined by puromycin reactivity. (C) control; Δ, ΔSRL. A representative experiment is shown on the left, and results of three independent experiments (mean ± SEM) are shown on the right. (*) P < 0.05, (ns) not significant. (B,C) Measures of EF-Tu-dependent decoding in control and mutant 70S ICs, as indicated. (B) Representative raw data showing fMet-Tyr formation as a function of time. (C) Plots of fMet-Tyr formation versus time, for various 70S ICs, as indicated in the key. Data points represent mean ± SEM values of three independent experiments. In some cases, error bars are smaller than (and hidden by) the symbols. Curves represent single-exponential fits of the data. (D) Data from analogous reactions that lack EF-Tu.
