The KD values of binding between IFIT1 and 5′ RNA cap analogs or 5′ capped RNAs
| 5′ RNA cap analog | Capped  KD (nM)
|
Capped  C.I. (nM)
|
Trinucleotide capbKD (nM) | Trinucleotide capd C.I. (nM) |
|---|---|---|---|---|
| m7GpppApG | 100 | (60, 170) | 100 | (60, 150) |
| m7GpppAmpG | 520 | (350, 770) | 380 × 103 | (190, 610) × 103 |
| m7GpppCpG | 230 | (150, 360) | 13 × 103 | (10, 15) × 103 |
| m7GpppGpG | 330 | (220, 480) | 23 × 103 | (14, 39) × 103 |
| m7GpppUpG | 130 | (70, 250) | 390 × 103 | (330, 440) × 103 |
| m7Gpppm6ApG | 230 | (140, 370) | 50 × 103 | (20, 100) × 103 |
| m7Gpppm6AmpG | 900 | (730, 1100) | 380 × 104 | (210, 780) × 104 |
| GpppApG | 11 | (5, 20) | 370 × 104 | (210, 660) × 104 |
| NADpG | 1000 | (800, 1400) | 430 × 106 f | (200, 540) × 106 |
 GpppApG
|
23 | (10, 46) | 30 | (19, 47) |
| FADpG | 80 | (60, 110) | 21 × 103 | (13, 32) × 103 |
| pppUpG | 270 | (200, 370) | 26 × 106 | (14, 112) × 106 |
| Glc-ppUpG | 8200 | (6200, 11,100) | n/be | n/be |
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aConditions: 20 mM HEPES (pH 7.5), 200 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments.
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bConditions: 20 mM HEPES (pH 7.5), 100 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments.
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cRNA sequences is RNA III shown in Figure 3 and given in the Experimental section.
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dC.I. (confidence interval) values estimated automatically by PALMIST- ESP procedure (Error Surface Projection). Confidence level set at 68.3% (equivalent to ±1σ in Gaussian bell curve).
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e(n/b) No binding was observed up to concentration of 40 mM ligand.
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fA well-behaved fit for these data was not obtained.
