TABLE 2.
The cap-1:cap-0 KD values ratio for all tested sequence variants
| KD cap-1/KD cap-0 | RNA Ia | RNA IIa | RNA IIIa | RNA IVa,c | Trinucleotideb |
|---|---|---|---|---|---|
| cap-1/cap-0 | 4.2 | 4.0 | 5.2 | 27.3 | 3800 |
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aConditions: 20 mM HEPES (pH 7.5), 200 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments with confidence intervals (C.I.).
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bConditions: 20 mM HEPES (pH 7.5), 100 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments with confidence intervals (C.I.).
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cRNA sequences are shown in Figure 3 and Supplemental Table S1.
