TABLE 1.
Binding affinities of IFIT1 determined by MST for cap-0 and cap-1 structures depending on the RNA sequence
| KD (C.I.)/(nM) | |||||
|---|---|---|---|---|---|
| Cap structure | RNA Ia,c | RNA IIa,c | RNA IIIa,c | RNA IVa,c | Trinucleotideb |
| m7GpppApG | 8 (1–19) | 15 (8–25) | 100 (60–170) | 150 (90–230) | 100 (60–150) |
| m7GpppAmpG | 34 (19–59) | 60 (42–84) | 520 (350–770) | 41 (26–70) ×102 | 380 (190–610) ×103 |
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aConditions: 20 mM HEPES (pH 7.5), 200 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments with confidence intervals (C.I.).
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bConditions: 20 mM HEPES (pH 7.5), 100 mM NaCl, 0.075% Pluronic F-127, mean values from triplicate experiments with confidence intervals (C.I.).
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cRNA sequences are shown in Figure 3 and Supplemental Table S1.
