TABLE 2.
Growth parameters for E. coli expressing ksgA variants
| [Ksg] = 0 μg/mL Lag (h) |
Doubling time (h) | [Ksg] = 512 μg/mL Lag (h) |
Doubling time (h) | |
|---|---|---|---|---|
| Vector | 0.2 ± 0.1 | 0.52 ± 0.07 | 0.3 ± 0.2 | 0.8 ± 0.1 |
| Wt | 0.4 ± 0.4 | 0.4 ± 0.3 | 7 ± 1 | 0.4 ± 0.2 |
| Y116A | 2.8 ± 0.3 | 0.48 ± 0.05 | 2.7 ± 0.4 | 1.05 ± 0.05 |
| R12E R14E | 0.8 ± 0.7 | 0.4 ± 0.2 | 5 ± 2 | 1.2 ± 0.6 |
| K13E | 1.5 ± 0.2 | 0.53 ± 0.07 | 5.5 ± 0.4 | 1.2 ± 0.1 |
| G16D | 1.1 ± 0.8 | 0.5 ± 0.2 | 3.6 ± 0.4 | 0.9 ± 0.2 |
| Q17L | 0.8 ± 0.6 | 0.50 ± 0.05 | 0.8 ± 0.3 | 0.86 ± 0.08 |
| N18A | 1.7 ± 0.2 | 0.60 ± 0.04 | 2.4 ± 0.2 | 0.8 ± 0.2 |
| F19A | 1.54 ± 0.05 | 0.7 ± 0.1 | 1.8 ± 0.5 | 1.0 ± 0.2 |
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The mean and standard deviation for the length of lag phase in hours (h) and the growth rate during exponential phase in hours were obtained from curve fitting. One-way ANOVA was performed with wt (italics) as the reference. Values significantly different than wt are shown in bold, P ≤ 0.05.
