TABLE 1.
Single turnover kinetics and selected RNA affinity binding parameters
| kchem (min−1) | K1/2,SAM (μM) | Kd, RNA (μM) | |
|---|---|---|---|
| wt | 0.14 (0.092–0.23) | 4.2 (2.1–10) | 0.61 (0.50–0.74) |
| R36E | 0.033 (0.027–0.044) | 0.83 (0.38–1.8) | 0.89 (0.70–1.1) |
| F43A | 0.0061 (0.0040–0.013) | 2.6 (0.94–9.6) | 0.37 (0.31–0.43) |
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Parentheses indicate 95% confidence interval.
