Two dynamic N-terminal regions are required for function in ribosomal RNA adenine dimethylase family members

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FIGURE 8.
FIGURE 8.

The N-terminal basic region and motif X of KsgA contribute to 16S rRNA methylation in vitro. (A) KsgA catalyzed methylation of 16S rRNA in vitro was measured using 30S ribosomes devoid of m62A1518 and m62A1519 (ΔksgA strain) and a radiolabeled SAM cofactor. (B) Wt KsgA robustly methylates 30S ribosomes in vitro. The Y116A active site mutant is methylation deficient and serves as a negative control. (****) P ≤ 0.001.

This Article

  1. Published in Advance November 8, 2024, doi: 10.1261/rna.080068.124 RNA 31: 164-180

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