Two dynamic N-terminal regions are required for function in ribosomal RNA adenine dimethylase family members

Site-directed mutants in the N-terminal basic region and motif X are associated with altered phenotypes in ErmC. (A) An AlphaFold2 model of ErmC demonstrating the predicted location of basic region residues K4 and K7 and motif X residues S9-I13. (B) MIC values for erythromycin were measured for E. coli cells expressing ermC site-directed mutants in the basic region and motif X. MIC values were measured from three replicates. The range of MIC values observed is reported. Where one value is listed, all replicates possessed this value.