Two dynamic N-terminal regions are required for function in ribosomal RNA adenine dimethylase family members

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FIGURE 5.
FIGURE 5.

Single turnover kinetics. (A) Methylated RNA versus time plots for increasing concentration of wild-type (wt) ErmE and site-directed mutants. (B) Methylation rates (kobs) are plotted versus ErmE concentration under limiting SAM and excess RNA conditions and fit to a model for single-turnover kinetics to extract kchem and K1/2,SAM values. An inset is shown of the F43 data with a y-axis range of 0–0.006 min−1.

This Article

  1. Published in Advance November 8, 2024, doi: 10.1261/rna.080068.124 RNA 31: 164-180

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