FIGURE 3.
CSDE1 isoforms have similar intrinsic capacity to promote metastatic traits. (A) Scheme of the experimental workflow used to replace endogenous CSDE1 with its individual isoforms. (B) Representative western blot showing expression of endogenous and exogenous CSDE1 isoforms. Tubulin is shown as loading control. (C) Effects of CSDE1 isoform expression on anoikis resistance (left) and spheroid growth (right) in SK-Mel-147 cells. Boxplots show the results of two biological replicates, each with five technical replicates. Values are normalized to the average of the shCTRL + GFP samples. Significance was assessed by t-test (*P ≤ 0.05, **P ≤ 0.01).
