m⁶A methylation inhibits recruitment of the Dand5 3′UTR to the left–right determinant Bicc1

Murine node cells transcribe m⁶A writers, readers, and erasers. (A) UMAP plot colored by cell population of mouse embryos at embryonic day (E) 7.5 (left). The relative expression of specific genes of interest in each of these populations is depicted by the dot plot to the right. Colors, expression levels; dot size, fraction of cells expressing the gene indicated on the x-axis. (B) UMAP plot showing the relative expression levels of Foxj1, Bicc1, Dand5, and Nodal across the cell populations in A. (C) Violin plots of the expression levels of m⁶A writers, erasers, and readers in Foxj1⁺ Dand5⁺ (n = 57) and in Foxj1⁺ Dand5⁻ (n = 211) axial mesendoderm (axME) cells. Data were retrieved from a public scRNA-seq data set (Scheibner et al. 2021). Cells containing at least one unique molecular identifier (UMI) were considered to be positive for the transcript of interest.