m⁶A methylation inhibits recruitment of the Dand5 3′UTR to the left–right determinant Bicc1

Identification of an m⁶A methylation site in Dand5 3′UTR. Coverage of m⁶A-enriched reads along Dand5 mRNA (A) and its 3′UTR (B) in mouse testes. Light and dark blue curves show the m⁶A reads in m⁶A immunoprecipitates and in corresponding input fractions, respectively. The normalized coverages (in reads per million [rpm]) from three biological replicates are plotted separately. A methylated adenosine in position 3 of the conserved AGACGUGAC motif of the Dand5 3′UTR is marked by a blue arrow. Data were retrieved from m⁶A-IP-seq data set generated from adult mouse testes (Mendel et al. 2021).