Bioinformatics-driven refinement of the commonly used TPI nonsense-mediated decay reporter system

(q)RT PCR
#1224 TCTTCCAGCCTCCCATCAGCGTTTGG
#1225 CAACAGAAATCCAACCTAGAGCTGCT
#3502 CCACTCCTCCACCTTTGAC
#3503 ACCCTGTTGCTGTAGCCA
#4004 GCCGCATTTTTTAACCCT
#4005 CTCTCTCTTCTCCTATCTCT
#4324 TAATACGACTCACTATAGG
#5664 CCAGCACCATGGCG
#5665 CTCCTTGCAGGTTGCC
#5736 ATGCCAGGGCTGATCTC
#7188 GCCCAATCAGCGGTGTC
Cloning
#5607 GGTTGCTCCCTGGAGAATG
#5665 CTCCTTGCAGGTTGCC
#5672 GAGTCAGATGAGGTAAGTAGCCAAG
#5673 CCCAAAGACATGCCTTCTCTCTG
#5682 AGATCCCAAGATTGCTGTGGCTGCGCAG AACTGCTACAAAGTGACTA ATGGGGCTTTTACTGGAGAGATCAGGTG AGATCTAGGCGGAGAGGGGTGTGTGG
#5683 TCTCCGCCTAGATCTCACCTGATCTCTCCAGTAAA
#5743 GAGCTCATCGGCACTCT
#5744 CTATAGGCTAGCGCCTCGGCTCCAGCACCATGG CGGAGGACGGC

This Article

  1. Published in Advance October 16, 2024, doi: 10.1261/rna.080134.124 RNA 31: 32-42

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  1. July 2026, 32 (7)
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