The adjusted variants of the reporter plasmids produce single bands upon treatment with different inhibitors. RT-PCR results of HeLa cells transiently transfected with 1 µg of the adjusted pCI-TPI-WT or PTC plasmid for 24 h that were either untreated (lanes 1, 3, 5, and 7) or treated with 50 µg/mL CHX for 6 h (lanes 2 and 4) or 10 mM caffeine for 4 h (lanes 6 and 8), respectively. The genes of interest were amplified with specific primer pairs (pTPI: #4324/#5665 18 cycles, HGH transfection control #1224/#1225 26 cycles, NMD control SRSF3 #4003/#4004 35 cycles), separated on a 10% PAA gel and visualized by EtBr staining and UV light exposure.
