The pTPI splice isoforms are independent of Intron 1 and ATG alterations. RT-PCR results of HeLa cells transiently transfected with 1 µg of the pCI-TPI-WT plasmid or altered constructs for 24 h that were either untreated (lanes 1, 3, and 5) or treated with 50 µg/mL CHX for 6 h (lanes 2, 4, and 6). The genes of interest were amplified with specific primer pairs (pTPI: #4324/#5665, 18 cycles, HGH transfection control #1224/#1225, 26 cycles, NMD control SRSF3 #4003/#4004, 35 cycles), separated on a 10% PAA gel and visualized by EtBr staining and UV light exposure. The pTPI transcripts were amplified with specific primer pairs (#4324/#5665, 26 cycles), HGH served as transfection control (#1224/#1225, 26 cycles). The PCR products were separated on a 10% PAA gel and visualized by EtBr staining and UV light exposure.
