S1 analysis of specific transcripts confirms specific roles for the Ccr4-Not complex subunits in expression of mRNAs. Total cellular RNA was extracted from the indicated wild-type and mutant strains grown to exponential phase or 30 min after glucose depletion (post-diauxic) as indicated, and 30 µg were analyzed by S1 digestion for the levels of the indicated mRNAs in the different indicated strains. All hybridizations were internally controlled by cohybridization with DED1 and all hybridizations with a given probe were analyzed on the same gel (Supplemental Fig. 4) even though not always in the same order, such that in the figure signals for each strain have been in some cases cut and pasted such that the order for each of the strains is in relatively the same position. Black lines demonstrate that bands were cut and combined from different blots.
