Corrigendum: Specific roles for the Ccr4-Not complex subunits in expression of the genome
The Figure 2 legend in the above-mentioned article includes the following text: “…all hybridizations with a given probe were analyzed on the same gel even though not always in the same order, such that in the figure signals for each strain have been in some cases cut and pasted such that the order for each of the strains is in relatively the same position.” The authors have been made aware that the juxtaposition of lanes in Figure 2 that were not juxtaposed in the original blots is difficult to interpret critically without providing the original blots. Please note that the conclusions drawn from the data have not changed.
The revised Figure 2 (below) has been consolidated by separating each lane with a line to indicate, as was already described in the Figure 2 legend, that the lanes were not juxtaposed in the original blots, and the original blots have now been provided as raw data files (Supplemental Fig. 4, below). The revised legend is also provided.
The conclusions drawn from the data have not changed, and the authors apologize for any confusion this may have caused.
doi:10.1261/rna.080301.124
S1 analysis of specific transcripts confirms specific roles for the Ccr4-Not complex subunits in expression of mRNAs. Total cellular RNA was extracted from the indicated wild-type and mutant strains grown to exponential phase or 30 min after glucose depletion (post-diauxic) as indicated, and 30 µg were analyzed by S1 digestion for the levels of the indicated mRNAs in the different indicated strains. All hybridizations were internally controlled by cohybridization with DED1 and all hybridizations with a given probe were analyzed on the same gel (Supplemental Fig. 4) even though not always in the same order, such that in the figure signals for each strain have been in some cases cut and pasted such that the order for each of the strains is in relatively the same position. Black lines demonstrate that bands were cut and combined from different blots.
Raw data used to make Figure 2. S1 analysis of specific transcripts confirms specific roles for the Ccr4-Not complex subunits in expression of mRNAs. Total cellular RNA was extracted from the indicated wild-type and mutant strains grown to exponential (E) phase or 30 min after glucose depletion (PD) as indicated, and 30 µg were analyzed by S1 digestion for the levels of the indicated mRNAs in the different indicated strains. All hybridizations were internally controlled by cohybridization with DED1.
