Summary of E. coli A49 suppressor strains isolated from this study
| Straina | Descriptiona | Relevant genotypeb | Other mutations | Mean growth rate (h−1) ± SDc | Mucoid at 30°Cd | |
|---|---|---|---|---|---|---|
| 30°C | 40°C | |||||
| DA5438 | rnpA (WT) | rnpA | – | 1.27 ± 0.14 | 2.24 ± 0.11 | – |
| DA61546 | rnpA49 (A49 parental) | rnpA49 | – | 0.97 ± 0.06 | 0.39 ± 0.15 | – |
| DA64107 | 3× rnpA49 | rnpA49, DUP(3737267..3928428) | – | 1.11 ± 0.05 | 1.05 ± 0.12 | – |
| DA64110 | 2× rnpA49 | rnpA49, DUP(3844972..3918184) | lsrA[A451A] (GCA > GCT) (synonymous) | 1.20 ± 0.05 | 0.98 ± 0.12 | – |
| DA65198 | 5× rnpA49 | rnpA49, DUP(3868817..389802) | – | 1.18 ± 0.01 | 1.25 ± 0.03 | – |
| DA65199 | 2× rnpA49 | rnpA49, DUP(3804071..3899637) | – | 1.17 ± 0.17 | 1.17 ± 0.15 | – |
| DA65187 | 16× rnpB | rnpA49, DUP(3251561..3286079) | insA[T24T] (ACT > ACC) (synonymous) | 1.24 ± 0.02 | 1.24 ± 0.11 | – |
| DA65194 | 7× rnpB | rnpA49, DUP(3267203..3284424) | – | 1.12 ± 0.08 | 1.71 ± 0.01 | – |
| DA64106 | rnr::Δ10 bp | rnpA49, rnr::DEL(4404082..4404091) | – | 0.98 ± 0.01 | 0.51 ± 0.06 | – |
| DA65197 | rnr::IS1 | rnpA49, rnr::IS1 | trmA::IS186 | 0.92 ± 0.02 | 0.68 ± 0.02 | – |
| DA74911 | Δrnr::kanR (control) | rnpA49, DELrnr::kanR | – | 0.81 ± 0.01 | 0.54 ± 0.02 | – |
| DA74851 | ΔpcnB::kanR (control) | rnpA49, DELpcnB::kanR | – | 0.81 ± 0.29 | 0.61 ± 0.13 | – |
| DA64111 | lon::IS186 | rnpA49, lon::IS186 | – | 1.20 ± 0.02 | 0.87 ± 0.05 | ++ |
| DA64113 | lon::IS186 | rnpA49, lon::IS186 | – | 1.22 ± 0.01 | 0.81 ± 0.11 | + |
| DA65192 | lon[C39F] | rnpA49, lon[C39F] (TGT > TTT) | – | 1.20 ± 0.01 | 0.97 ± 0.03 | – |
| DA65193 | lon[C39Y] | rnpA49, lon[C39Y] (TGT > TAT) | – | 1.16 ± 0.02 | 0.98 ± 0.04 | + |
| DA65200 | lon[P63S] | rnpA49, lon[P63S] (CCG > TCG) | – | 1.18 ± 0.06 | 1.03 ± 0.02 | – |
| DA64108 | lon[Q225P] | rnpA49, lon[Q225P] (CAG > CCG) | – | 1.20 ± 0.05 | 1.04 ± 0.09 | – |
| DA64112 | lon[L230R] | rnpA49, lon[L230R] (CTG > CGG) | – | 1.25 ± 0.03 | 0.95 ± 0.09 | + |
| DA64109 | lon[E240G] | rnpA49, lon[E240G] (GAA > GGA) | – | 1.30 ± 0.03 | 1.00 ± 0.05 | ++ |
| DA65195 | lon[E240K] | rnpA49, lon[E240K] (GAA > AAA) | – | 1.25 ± 0.01 | 1.06 ± 0.03 | + |
| DA73974 | Δlon::kanR (control) | rnpA49, DELlon::kanR | – | 1.27 ± 0.02 | 1.00 ± 0.05 | +++ |
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aDA5438 is E. coli MG1655 carrying the wild-type rnpA allele; DA61546 is the E. coli A49 parental strain carrying the ts rnpA49 allele. Relevant control strains containing kanR deletion cassettes from the KEIO collection in the parental DA61546 A49 background are included for comparison. For strains containing amplifications, the relevant gene and its estimated copy number are given.
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b“DUP” indicates strains containing genomic amplifications; the genomic coordinates for the boundaries of the amplified units are given. “DEL” indicates strains containing deletions; the genomic coordinates for the boundaries flanking the deletion are given. For complete strain genotypes, see Supplemental Table S1. Genomic coordinates are relative to the E. coli MG1655 reference genome (GenBank: U00096.3).
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cExponential phase growth rates of each strain in LB medium at both 30°C (permissive temperature) and 40°C (sublethal temperature); the values reported represent the mean of at least three independent biological replicates (with two technical replicates each) ± the standard deviation of the mean.
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dNot mucoid at 30°C (−) or mucoid at 30°C (+ < ++ < +++), as observed on LB-agar plates after overnight incubation.
