FIGURE 5.
Luc7 ZnF2_L/L2 suppresses the cs phenotype of Prp28 R499A. (A) Schematic showing the impact of Prp28 alleles that modulate the U1 to U6 exchange during splicing. Truncation of Luc7 bypasses the requirement for Prp28 in splicing and allows splicing to proceed whereas Prp28 ATPase mutants inhibit this step and stall splicing. (B) Luc7 ZnF2_L/L2 does not bypass the requirement for Prp28 in splicing unlike Luc7 NΔ31. “-HW” and “-HW + FOA” refer to synthetic media lacking histidine and tryptophan in the absence or presence of 5-fluoroorotic acid, respectively. (C) Luc7 ZnF2_L/L2 suppresses cold sensitivity associated with Prp28 R499A.
