Functional analysis of the zinc finger modules of the Saccharomyces cerevisiae splicing factor Luc7

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FIGURE 2.
FIGURE 2.

Yeast are viable with humanized Luc7 ZnF1 and ZnF2. (A) Luc7 ZnF1 mutants are viable in yeast. The ZnF2_L/L2 mutant is also viable, whereas ZnF2_L3 is lethal. (B) Expressing Luc7 with mutations in both ZnF1 and ZnF2 does not rescue the lethality associated with ZnF2_L3. “-W” and “-W + 5-FOA” refer to synthetic media lacking tryptophan and media lacking tryptophan and containing 5-fluoroorotic acid, respectively.

This Article

  1. Published in Advance May 7, 2024, doi: 10.1261/rna.079956.124 RNA 30: 1058-1069

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