Conservation of acp3U and m7G Escherichia coli and Pseudomonas aeruginosa. Heatmap of average (from three independent samples cultured in CDM) frequency of misincorporation and termination score at positions 46 and 47 in tRNA from E. coli (left) and P. aeruginosa (right). Only tRNAs with read coverage >100 at position 46 and an identified isoacceptor homolog are represented. Known modifications for E. coli are represented to the right of the E. coli heatmap. The short name for each modification is used (Supplemental Table S11). Sequences in P. aeruginosa at positions 46 and 47 are represented to the right of the P. aeruginosa heatmap. The absence or presence of a signature is indicated based on a 5% cutoff of both misincorporation and termination for positions 46 or 47 by circle marks. The key is listed below the heatmap.
